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Cellscript Inc scriptcap m7g capping system
Scriptcap M7g Capping System, supplied by Cellscript Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/scriptcap+m7g+capping+system/1+cap+capping+scriptcaptm+system/bio_rxiv__64898__2026__04__28__721236-62-15-19
Average 86 stars, based on 1 article reviews
scriptcap m7g capping system - by Bioz Stars, 2026-10
86/100 stars

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Related Articles

Luciferase:

Article Title: Translation of zinc finger domains induces ribosome collision and Znf598-dependent mRNA decay in zebrafish.
Article Snippet: Bright-field images were acquired using a SteREO Lumar V12 microscope and an AxioCam MRc camera with ZEN software (Zeiss, Jena, Germany) in spline mode. .. Myc-tagged Znf598 mRNAs and tandem ORF luciferase mRNAs were transcribed from a linearized plasmid DNA template using the mMESSAGE mMACHINE SP6 Transcription Kit (Thermo Fisher Scientific) and purified using the RNeasy Mini Kit (QIAGEN). sfGFP, Myc-tagged EGFP, and Myc-tagged Znf236 mRNAs were transcribed from a linearized plasmid DNA template using the SP6-Scribe Standard RNA IVT Kit (CELLSCRIPT), the m7G cap structure was added to the purified RNA using the ScriptCap m7G Capping System (CELLSCRIPT), the poly(A) tail was added using the A-Plus Poly(A) Polymerase Tailing Kit (CELLSCRIPT), and the mRNA was purified using the RNeasy Mini Kit. ..

Plasmid Preparation:

Article Title: Translation of zinc finger domains induces ribosome collision and Znf598-dependent mRNA decay in zebrafish.
Article Snippet: Bright-field images were acquired using a SteREO Lumar V12 microscope and an AxioCam MRc camera with ZEN software (Zeiss, Jena, Germany) in spline mode. .. Myc-tagged Znf598 mRNAs and tandem ORF luciferase mRNAs were transcribed from a linearized plasmid DNA template using the mMESSAGE mMACHINE SP6 Transcription Kit (Thermo Fisher Scientific) and purified using the RNeasy Mini Kit (QIAGEN). sfGFP, Myc-tagged EGFP, and Myc-tagged Znf236 mRNAs were transcribed from a linearized plasmid DNA template using the SP6-Scribe Standard RNA IVT Kit (CELLSCRIPT), the m7G cap structure was added to the purified RNA using the ScriptCap m7G Capping System (CELLSCRIPT), the poly(A) tail was added using the A-Plus Poly(A) Polymerase Tailing Kit (CELLSCRIPT), and the mRNA was purified using the RNeasy Mini Kit. ..

Purification:

Article Title: Translation of zinc finger domains induces ribosome collision and Znf598-dependent mRNA decay in zebrafish.
Article Snippet: Bright-field images were acquired using a SteREO Lumar V12 microscope and an AxioCam MRc camera with ZEN software (Zeiss, Jena, Germany) in spline mode. .. Myc-tagged Znf598 mRNAs and tandem ORF luciferase mRNAs were transcribed from a linearized plasmid DNA template using the mMESSAGE mMACHINE SP6 Transcription Kit (Thermo Fisher Scientific) and purified using the RNeasy Mini Kit (QIAGEN). sfGFP, Myc-tagged EGFP, and Myc-tagged Znf236 mRNAs were transcribed from a linearized plasmid DNA template using the SP6-Scribe Standard RNA IVT Kit (CELLSCRIPT), the m7G cap structure was added to the purified RNA using the ScriptCap m7G Capping System (CELLSCRIPT), the poly(A) tail was added using the A-Plus Poly(A) Polymerase Tailing Kit (CELLSCRIPT), and the mRNA was purified using the RNeasy Mini Kit. ..

Article Title: Target cleavage and gene silencing by Argonautes with cityRNAs.
Article Snippet: Unincorporated g-32P ATPwas removed using MicroSpin G-25 columns (Cytiva). .. In vitro transcribed 58-60-nt targets were cap-labeled with a-32P-GTP (3,000 Ci mmol 1; PerkinElmer) using ScriptCap m7G Capping System (CellScript) and purified with MicroSpin G-50 columns (Cytiva). ..

Generated:

Article Title: TALEN-mediated intron editing of HSPCs enables transgene expression restricted to the myeloid lineage.
Article Snippet: TALEN pair used for editing the AAVS1 locus targeted 50 – CCCCTCCACCCCACA – 30 and 50 – GTCACCAATCCTGTC – 30 (Figure S6A). mRNA for most experiments was purchased from TriLink BioTechnologies (San Diego, CA, USA). .. For AAVS1 TALEN mRNA, some in-house productions were generated using the T7-FlashScribe Transcription Kit combined with the ScriptCap m7G Capping System and the A-Plus Poly(A) Polymerase Tailing Kit (CellScript, Madison, WI, USA) according to the manufacturers’ protocols. mRNA quality was assessed via Fragment Analyzer (Agilent Technologies). .. AAV donor plasmid construction and production AAV6 donor sequences used to edit the CD11b and AAVS1 loci with eGFP or IDUA inserts (Figure S6B) were designed using Geneious software and ordered from GenScript (Piscataway, NJ, USA).

In Vitro:

Article Title: Target cleavage and gene silencing by Argonautes with cityRNAs.
Article Snippet: Unincorporated g-32P ATPwas removed using MicroSpin G-25 columns (Cytiva). .. In vitro transcribed 58-60-nt targets were cap-labeled with a-32P-GTP (3,000 Ci mmol 1; PerkinElmer) using ScriptCap m7G Capping System (CellScript) and purified with MicroSpin G-50 columns (Cytiva). ..

other:

Article Title: Target cleavage and gene silencing by Argonautes with cityRNAs
Article Snippet: ScriptCap TM m7G Capping System , Cellscript.LLC. , Cat# C-SCCE06245.



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